Non-Alcoholic Extraction of Rose Pigment as a Halal and Safe Natural Colorant and Bioactive Compound

Rose is a well-known cut ornamental flower. The rose crown has been known to contain high anthocyanin, particularly cyanidine and dephinidine-glycoside. Those bioactive compounds have been proven to act as free-radical scavengers. Low grade and non-fresh flowers still contains potential pigments, which can be utilized as natural colorant for food and supplement such as effervescent tablet. The study was to investigate the effect of post-cutting process on the quality of anthocyanin extract and the quality products, and to examine bioactivity of rose effervescent tablets on the liver protection through SGPT test using white rat. The study consists of three experiments. First experiment was aimed to investigate the effect of pigment quality derived from rose crown, i.e. fresh cut rose, 2 and 4 days after cutting. Further, the pigment was then applied on several products (i.e. fruit juice, jelly and carbonated drink or effervescent tablet) and the qualities were examined in second experiment. Lastly, the third experiment deals with pre-clinical examination using white rats.The study showed that anthocyanin pigment of rose in the dosage of 3% significantly contributed to formation of a red and yellow color in those industrial products. Effervescent tablet derived from local rose significantly decreased SGPT level of white rat wistar strain induced by CCl4.

References: 

Agustin, I.R. (2009). Study of making effervescent tablet using extracts of red roses (Rosa damascena Mill) (Assessment Flower Color and Type Sugar). Thesis. Department of Food Science and Technology, University of Muhammadiyah Malang. Malang:-Indonesia (Studi pembuatan tablet effervescent dengan menggunakan ekstrak bunga mawar merah (Rosa damascena Mill) (Kajian Warna Bunga dan Jenis Gula).

Anonim. (2010). Corn sugar: (Gula jagung). Retrieved on April 5, 2010 from: http://www.Gula jagung.com.

AOAC. (1995). Official method of analysis. 16th edition. Chapter 37, Fruit and fruit product. Association of Official Analytical Chemistry International. Gaithersburg.

Blake, S. (2004). Medicinal Plant names-Sample excerpt. Retrieved on August 12, 2010 from: http://www.naturalhealthwizards.com/MedicinalPlantNamesSample.pdf.

Budiarto, H. (1991). Anthocyanin stability Gurcuna Mangostanan in carbonated beverages. Thesis Department of Food Technology Nutrition. Bogor Agricultural Institute of Technology: (Stabilitas Antosianin Gurcuna Mangostanan dalam Minuman Berkarbonat. Skripsi Jurusan Teknologi Pangan Gizi. Institut Teknologi Pertanian Bogor).

Downey, M.O, N.K. Dokoozlian, M.P. Krstic. (2006). Cultural practice and environmental impacts on the flavonoid composition of grapes and wine: A review of recent research. Am. J. Enol. Vitic. 57:3:257-268.

Fabre, C.E., A.L. Santerre, N.O. Loret, R. Baberian, A. Parailleux, G. Goma, P.J. Blanc, (1993). Production and food applications of the red pigments of Monascus rubber. J. Food Sci. 58 (5): 1099-1110.

Fennema, O.R. (2000). Food chemistry. Marcel Dekker. New York.

Garz’on, G.A., K.M. Riedi, S.J. Schwartz, (2009). Determination of anthocyanins, total phenolic content, and antioxidant activity in Andes Berry (Rubus glaucus Benth). J. Food Sci. 74 (3):227-232.

Harborne, J.B. (1987). Phytochemical methods: guide how to analyze modern plant. Bandung Institute of Technology: (Metode fitokimia: Penuntun cara modern menganalisa tumbuhan. Institut Teknologi Bandung. Bandung).

Henry, G. A. F, J.O. Houngton. (1996). Natural food colorants. Second Edition. Blackie Academic and Professional. London.

Jenie, B.S.L., Helianti, S. Fardiaz. (1994). Utilization of tofu dregs, onggok and bran for red pigment production by Monascus purpureus. Bulletin of Food Technology and Industry (5): 22-29: (Pemanfaatan ampas tahu, onggok dan dedak untuk produksi pigmen merah oleh Monascus purpureus. Buletin Teknologi dan Industri Pangan (5): 22–29.

Kompas. (2009). BPOM tarik 70 produk kosmetik berbahaya. Editor: Rosdianah Dewi. Jakarta, June 11, 2009.

Konga, J.M., L.S. Chiaa, N.K. Goha, T.F. Chiaa, R. Brouillard. 2007. Analysis and biological activities of anthocyanins. Abstracts. National Institute of Education, Nanyang Technological University, Singapore; and Institut de Chimie, Universite Louis Pasteur, France

Lewis R.J. (1993). Hawley’s condensed chemical dictionary. 22th Editions. Van Nostrand Reihold Company. New York.

Lewis, D.H., Bloor, S.J. and Mitchell, K.A.(1997). Flower colour in cymbidium, what makes up the colour you see ?. Retrieved from: http://www.crop.cri/nz/meidakit/Release/971308835.html.

Li, J. (2009).Total anthocyanin content in blue corn cookies as affected by ingredients and oven types. Dissertation. Department of Grain Science and Industry. College of Agriculture. Kansas University. Manhattan, Kansas. pp 111.

Lopes, D., J. Dettmann, C. Nimalaratne, A. Schieber. (2010). Characterization and quantification of polyphenols in amazon grape (Pourouma cecropiifolia Martius). J. Molecules, 15: 8543-8552.

Madhavi, D.L., S.S. Desphpande, D.K. Dalunkhe, (1996). Food antioxidants, technological, toxicological and health perspectives. IV Series. Marcel Dekker, Inc. New York-Bassel-Hongkong.

Markakis, P. (1982). Anthocyanins as food colors. Academic Press. New York. pp 263.

Miyake, Y., K. Yamamoto, N. Tsujihara, T. Osawa. 1998. Protective effect of lemon flavonoids on oxidative stress in diabetic rats. Lipid, 33: 689-695.

Mohre, R. (1990). Effervescent tablets. In: Lachman, L., H.A. Lieberman, J.B. Schwartz. (Eds.). Pharmaceutical dosage forms: tablets (Vol. 2). Marcel Dekker. New York.

Nollet, L.M.L. (1996). Hand book of food analysis. New York: Marcel Dekker.

Rachmawati, Y. (2003). Effect of Meniran (Phyllantusniruri Linn) decoction on kidney glomerulus Rat (Rattus norvegicus) Wistar Strain Induced by CCl4. Not posted of Skripsi. Faculty of Medical, University of Brawijaya Malang: (Efek Pemberian Dekok Meniran (Phyllantusniruri Linn) terhadap Glomerulus Ginjal Tikus (Rattusnorvegicus) Strain Wistaryang Diinduksi CCl4. Skripsi Tidak Dipublikasikan. Malang: Fakultas Kedokteran UB.

Ramirez-Tortosa, C., Ø.M. Andersen, P.T. Gardner, P.C. Morrice, S.G. Wood, S.J. Duthie, G.G. Duthie. (2001). Anthocyanin-rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E-depleted rats. Free Radical Biology and Medicine31(9), 1033-1037.

Rukmana, R. (1995). Roses. Canisius. Yogyakarta: (Mawar. Penerbit Kanisius. Yogyakarta).

Saati, E.A., M. Wachid. (2006). Using anthocyanin pigments of sorting/not good Rose’s for natural colorant and inhibiting fat in Food Fermentation at Damage. Presented at the National Seminar PATPI 2 to 3 August, 2006, in the Faculty of Agricultural Technology-Univeristy Gajah Mada, Yogyakarta: (Penggunaan Pigmen Antosianin Bunga Mawar Sortiran untuk Pewarna dan Penghambat Kerusakan Lemak pada Pangan Fermentasi. Disampaikan pada Seminar Nasional PATPI 2-3 Agustus 2006, di FTP UGM, Yogyakarta).

Saati, E. S., Mujianto, R.E. Susestyarini. (2007). Function optimalization extract flower of kana (Rosa damascena Mill) as natural colourant and antioxidant by method isolating and characterizing the pigment. Reports Fundamental Research (Year I-II). DP3M-Higher Education National Education, Jakarta, pp 34-40: (Optimalisasi Fungsi Ekstrak Pigmen Bunga Mawar Merah (Rosa damascena Mill) sebagai Zat Pewarna dan Antioksidan Alami melalui Isolasi dan Karakterisasi. Laporan Fundamental Research (Tahun I-II). DP3M-DIKTI DIKNAS, Jakarta, hal 34-40).

Saati, E.A., Siti Z., Mujianto. (2008). Function optimization extract flower of kana as natural colorant and antioxidant by method isolating and characterizing the pigment. Proceeding International Research and Exhibition, UMM , November 7-8, 2008.

Saati, E.A. (2009). Patent registration, title of the invention: Effervescent Tablets of Extract Pigment Red Roses (Rosa sp.) and the manufacturing process. It was financed by Directorate of Research and Community Services (DIKTI-Republic of Indonesia: (Pendaftaran paten, judul invensi: Tablet Effervescent dari Ekstrak Pigmen Bunga Mawar merah (Rosa sp.) dan proses Pembuatannya. Dibiayai DP2M DIKTI Tahun 2009).

Saati, E.A. (2011). The anthocyanin pigment of red rose flower as potential natural colorant. Proceeding WHR 2011 Nominator Poster Presentations in Halal Science and Research Excellence, Kuala Lumpur, April 6-7, 2011.

Soni, K.A., G. Maria (2007). Antioxidantherbs present in fairness cream protects the skin from premature ageing. Journal of Chinese Clinical medicine 3: 5- 14.

Sudarmadji, S., Haryono, B., Suhardi. (1998). Analysis of food and agricultural materials. Liberty and Laboratory center of University of Gajah Mada. Yogyakarta: (Analisa Bahan Makanan dan Pertanian. Liberty dan Pusat Antar Universitas Gajah Mada. Yogyakarta).

Tranggono. (1990). Ingredients for Food Supplement (Food Additive). Laboratory center for Food and Nutrition, University of Gadjah Mada. Yogyakarta: (Bahan Makanan Tambahan (Food Additive). Pusat Antar Universitas Pangan dan Gizi Universitas Gadjah Mada. Yogyakarta).

Wu, S., C. FordG. Horn. (2009). Patent application number: 20090246343: Stable Natural Color Process, Products and Use Thereof.

Yue X., Z. Xu. (2008). Changes of anthocyanins, anthocyanidins, and antioxidant activity in Bilberry extract during dry heating. J. Food Chem.73 (6): 124-135.